製品の主な仕様
測定方法: 間接 ELISA 法
測定波長: 450 nm
測定試料: 血清、唾液、喀痰
交差性: Human
保管温度: 2-8℃保管
品質保証期限: 本品の有効期限は製造後 12 カ月です。
プレートへの固相化抗原: SARS-CoV-2 スパイクタンパク(S1ドメイン, 大腸菌発現系)
キット内容
1. SARS-CoV-2 スパイクタンパク (S1) 固相化プレート
2. HRP 標識抗ヒト IgA 抗体
3. 洗浄液 (10× PBST)
4. ウシ血清アルブミン (Diluent 用)
5. Diluent 用バッファー (10×)
6. R-1:発色基質 (TMB)
7. R-2:反応停止液 (1 mol/L 塩酸)
8. マイクロプレートシール
×1
10 μL×1
100 mL×1
×1
50 mL×1
22 mL×1
22 mL×1
×2
測定原理
-
固相化プレート上のリコンビナント SARS-CoV-2 スパイク(S1)タンパクと、これを認識する試料中の抗体 (以後、抗スパイクタンパク抗体と略称) を反応させる。
-
反応後、試料を洗浄操作により除去する。
-
固相化プレート上の抗スパイクタンパク抗体と、HRP (Horseradish peroxidase) 標識抗ヒト IgA 抗体を反応させる。
-
余剰の HRP 標識抗ヒト IgA 抗体を洗浄操作により除去する。発色基質を加え、吸光度を測定する。
測定の意義
本キットは、COVID-19 の原因ウイルスである、SARS-CoV-2 のスパイクタンパク(S1)を認識するヒト IgA 抗体を検出するための研究用 ELISA キットです。
ウイルスの検出には、大きく分けて二種類のアプローチ法があり、PCR 法などのウイルス自体を検出する方法と、感染時に発現する免疫反応(IgG, IgM, IgA)を検出する方法があります。IgA は唾液や鼻咽頭部などの粘膜部に特に多く存在し、一般的には感染を阻止する機能を持っています。
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